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Novel 3D Bioprinting Approach Generates Vascularized and Well-organized Cardiac Tissue Constructs

Advanced bioprinting technique employing iPSC-derived cardiomyocytes and endothelial cells generates cardiac tissue constructs with improved functionality

CRISPR/Cas9-based Exon Skipping Returns Dystrophin Expression to a Canine Model of DMD

Studies in an animal model of Duchenne muscular dystrophy highlight the in vivo utility of CRISPR/Cas9-based exon “skipping”-based treatment approaches

Homing CRISPR/Cas9 Technology to Barcode and Lineage Trace Mouse Development

A new CRISPR/Cas9 barcoding approach permits reconstruction of early mouse lineages and investigation of brain axis development

CRISPR Activation Strategy Takes Induced Pluripotent Stem Cell Generation to another Level

Targeting an embryo genome-activation motif permits the first generation of induced pluripotent stem cells via CRISPR activation of reprogramming factors

Three-Dimensional Bioprinting: The Future of Corneal Blindness Treatment?

Researchers employ pneumatic 3D extrusion bioprinting to generate bona fide biological corneal structures for regenerative medicine applications

CRISPR/Cas9-mediated in vivo Epigenetic Remodeling: A New Means to Treat Disease?

Researchers describe the application of CRISPR/Cas9-mediated epigenetic remodeling to treat kidney injury, diabetes, and muscular dystrophy in vivo

Computerized Colony Selection Promises to Increase iPSC Generation Efficiency

A new computerized technique based on morphological assessments may lead to increases in the efficiency of induced pluripotent stem cell reprogramming strategies 

Nanowarming Provides Big Results for Transplant Patients

A new nanoparticle-based technique for rewarming cryopreserved tissues may revolutionize the field of organ transplants

Creating Bioartificial Limb Replacements - Give us a hand!

Researchers describe the generation and transplantation of the first bioartificial complex limb graft via the repopulation of an extracellular matrix scaffold

It’s Now Easy to go with the Flow with Mouse Stem Cells - Cell-Surface Proteomics Identifies Lineage-Specific Markers of Embryo-Derived Stem Cells

From Developmental Cell
By Stuart P. Atkinson

Protocols for the reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) and the differentiation of pluripotent cells such as iPSCs and embryonic stem cells (ESCs) have taken great strides forward in recent times, increasing the efficiency and the quality of the desired cell product. However, the isolation of cells after reprogramming or differentiation is a major problem, as pure populations of cells are required to better understand such cells and also, more importantly, if they are to be used in for regenerative medicine. Isolation of cells is such a problem as there is a lack of validated cell-surface markers for flow cytometric analysis. However, researchers from the groups of Janet Rossant and Thomas Kislinger have analysed recently the cell surface proteomes of multiple mouse stem cell populations and have uncovered cell-surface protein patterns which allow for the unambiguous separation of different stem cell types from in vitro  and in vivo sources (Rugg-Gunn, Cox and Lanner).

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