From Developmental Cell
By Stuart P. Atkinson
Protocols for the reprogramming of somatic cells to induced pluripotent stem cells (iPSCs) and the differentiation of pluripotent cells such as iPSCs and embryonic stem cells (ESCs) have taken great strides forward in recent times, increasing the efficiency and the quality of the desired cell product. However, the isolation of cells after reprogramming or differentiation is a major problem, as pure populations of cells are required to better understand such cells and also, more importantly, if they are to be used in for regenerative medicine. Isolation of cells is such a problem as there is a lack of validated cell-surface markers for flow cytometric analysis. However, researchers from the groups of Janet Rossant and Thomas Kislinger have analysed recently the cell surface proteomes of multiple mouse stem cell populations and have uncovered cell-surface protein patterns which allow for the unambiguous separation of different stem cell types from in vitro and in vivo sources (Rugg-Gunn, Cox and Lanner).