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iPSC-model of SARS-CoV-2 Upper Airway Infection

A recent STEM CELLS study from the laboratory of Lyle Armstrong (Newcastle University, Newcastle upon Tyne, UK) reports on the development of a protocol to generate airway epithelial basal-like cells from induced pluripotent stem cells (iPSCs), which simplifies the manufacture of cellular models of the human upper airway. Djidrovksi et al. demonstrated that the culture of basal-like cells on transwell inserts and exposure to an air-liquid interface induced differentiation into a pseudostratified epithelial construct that can support SARS-CoV-2 infection/replication and the secretion of cytokines in a manner similar to that of in vivo airways.