Pluripotency marker expression after manipulation of microchamber geometry (height). Microchamber height was scaled to increase local volume and deplete concentrations of cell-secreted factors. Mouse embryonic stem cells cultured inside microfluidic chambers for 3 days showed decreased pluripotency and increased scattering phenotype with increasing chamber height. Please note that cartoon of microchambers is stylized. In real devices only the channel height was varied while the thickness of the roof was kept constant. Scale bar: 100 µm.
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Embryonic Stem Cells Cultured in Microfluidic Chambers Take Control of Their Fate by Producing Endogenous Signals Including LIF