Human Fibrinogen for Maintenance and Differentiation of Induced Pluripotent Stem Cells in Two Dimensions and Three Dimensions
Jarel K. Gandhi, et al., STEM CELLS Translational Medicine
As induced pluripotent stem cells approach clinical use for transplantation, the requirement for reagents that comply with regulatory practices needs to be addressed. To address this need, the use of human fibrinogen as a coating reagent for adherent culture of induced pluripotent stem cells was investigated. The data show that induced pluripotent stem cells cultured on fibrinogen maintain pluripotency, can be differentiated to the three germlayers, and can differentiate specifically to endothelial cells. As fibrinogen derived from human blood is readily available for clinical use, findings demonstrate an immediate solution to those developing regulatory-compliant cell therapy products for human application.
Survival/Adaptation of Bone Marrow-Derived Mesenchymal Stem Cells After Long-Term Starvation Through Selective Processes
Federico Ferro, et al., STEM CELLS
Mesenchymal stem cell (MSC) response to models of in vitro ischemia is of great interest for improving their survival and therapeutic efficacy. Understanding the effect of cell priming in serum-free medium, by concomitant starvation and culture in hypoxic conditions to induce their quiescence, would be of benefit to increase their survival rate when transplanted in vivo, as well as to enable the development of new improved culture protocols before MSC transplantation. MSC primed in this way may have the capacity not only to secrete trophic factors, increasing angiogenesis and reducing inflammation, but also to survive, fully integrate, and differentiate into the regenerating site.
Trial of intra‐articular injections of autologous adipose-derived MSCs in patients with knee osteoarthritis provides proof of functional improvement and pain relief
New findings suggest that the Sympk scaffold protein interacts with Oct4 to promote self‐renewal and pluripotency in mouse ESCs and preserve genome integrity
New research establishes senescent NPCs as a contributor to and potentially reversible factor in progressive MS while also suggesting MS as an aging-related disease