Despite the fact that malignant hematopoietic stem and progenitor cells play critical roles in the initiation, maintenance, and progression of acute myelogenous leukemia (AML) and myelodysplastic syndrome (MDS), information about the effects of investigational chemotherapeutic agents on these cells is limited. Using the Nedd8-activating enzyme inhibitor MLN4924 as an example, the authors describe a method for examining the impact of novel chemotherapeutics on these populations ex vivo, demonstrate that MLN4924 is generally more active against AML stem and progenitor cells than the standard-of-care agent cytarabine, and report that MLN4924 is also active against MDS stem and progenitor cells ex vivo.
In our study, we integrate diverse aspects of stem cell biology as the regeneration of parental stem cell diversity, the loss of stem cell properties with time in culture, and the control of gene expression at the promoter level by epigenetic marks in a multiscale computational model. Based on the assumption of bi-stabile epigenetic states, this model describes the broad and heterogeneous distribution of the stem cell antigen Sca-1 in mesenchymal stem cell clones. Moreover, it consistently explains both the fast regeneration of Sca-1 marker profiles and their long-term drifts with time in culture.
A new study in a myocardial infarction mouse model demonstrates how a novel reprogramming approach may permit enhanced cardiosphere-based myocardial regeneration
Video abstract from Drs. Barnawi, Al-Khaldi, Sleiman, Sarkar, Al-Dhfyan, Al-Mohanna, Ghebeh, and Al-Alwan on their recently published STEM CELLS paper entitled, "Fascin is Critical for the Maintenance of Breast Cancer Stem Cell Pool Predominantly via the Activation of the Notch Self-Renewal Pathway" Read the Paper here.
Video abstract from Dr. Allen on his recently published STEM CELLS paper entitled, "Angiopellosis as an Alternative Mechanism of Cell Extravasation" Read the Paper here.
Video abstract from Drs. Serena, Keiran, Ceperuelo-Mallafre, Ejarque, Fradera, Roche, Nuñez-Roa, Vendrell, and Férnandez-Veledo on their recently published STEM CELLS paper entitled, "Obesity and Type 2 Diabetes Alters the Immune Properties of Human Adipose Derived Stem Cells" Read the Paper here.
Confocal picture showing the myelinogenic potential of hiPS-NSCs engrafted at the border between the striatum and the corpus callosum of immunodeficient MLD mice at 3 months post-transplantation(green, human cells labeled with anti-human mitochondria; red, myelin basic protein; blue, nuclei counterstained with ToPro3). Scale bar: 100µm.
An ex vivo culture system used to isolate scalable quantities of hS/PCs with consistent expression of progenitor markers, is reported here. These hS/PCs can be maintained in long-term culture in 2D or in 3D, without loss of stem/progenitor markers. Incorporation of bioactive basement membrane-derived peptides in the 3D hyaluronate (HA) hydrogel culture system enhances progenitor expansion. Stimulation of hS/PC spheroids with neurotransmitter agonists leads to differentiation toward an acinar lineage.
Stem cells are emerging as a scientifically plausible treatment and possible cure for cerebral palsy, but are not yet proven. The lack of valid animal models has significantly hampered the scope of clinical trials. Despite the state of current treatment evidence, parents remain optimistic about the potential improvements from stem cell intervention and feel compelled to exhaust all therapeutic options, including stem cell tourism. Receiving unproven therapies from unvalidated sources is potentially dangerous. Thus it is essential that researchers and clinicians stay up to date. A systematic review and meta-analysis summarizing and aggregating current research data may provide more conclusive evidence to inform treatment decision making and help direct future research.